CRP ELISA Kit

Catalog No. ABIN8057098

The Rat CRP ELISA Kit (ABIN8057098) is a Colorimetric ELISA Kit designed to quantify Rat CRP.

Quick Overview for CRP ELISA Kit (ABIN8057098)

Target: CRP (C-Reactive Protein (CRP))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.13 pg/mL - 3000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details CRP ELISA Kit

Minimum Detection Limit: 3.13 pg/mL

Analytical Method: Quantitative

Specificity: Hs-CRP

Components:

• Plate
• Standard
• Diluent

Material not included: Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Protocol: This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat CRP.

Restrictions: For Research Use only

Handling

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: protect your eyes

Storage: 4 °C

Storage Comment: Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit

Target Details for CRP

Target: CRP (C-Reactive Protein (CRP))

Alternative Name: hs-CRP

Background: C-reactive protein (CRP) is an annular, pentameric protein. The physiological role of CRP is to bind to lysophosphatidylcholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via the C1Q complex. CRP is an acute phase protein in humans and is an important mediator of immune host defense. The acute phase response occurs in response to a wide range of acute and chronic inflammatory conditions such as bacterial, viral, or fungal infections, rheumatic and other inflammatory diseases, malignancy, and tissue injury and necrosis. These conditions cause release of IL-6 and other cytokines that trigger the synthesis of CRP and fibrinogen by the liver. CRP plays a role in innate immunity as an early defense system against infections. Monitoring of CRP levels in serum provides more detailed insights in several pathological situations such as inflammatory disease (including inflammatory bowel disease, Intestinal Lymphoma, Intestinal Tuberculosis and Behcet's Syndrome), cancer, diabetes, hypertension, cardiovascular disease, obstructive sleep apnea and so on.

Gene ID: 25419

NCBI Accession: NM_017096

UniProt: P48199

Pathways: Carbohydrate Homeostasis