PD-1 ELISA Kit

Catalog No. ABIN8070494

Human PD-1 ELISA Kit, Colorimetric assay for quantification of Human PD-1.

Quick Overview for PD-1 ELISA Kit (ABIN8070494)

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Binding Specificity: phosphorylated

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Cell Lysate, Tissue Lysate

Product Details PD-1 ELISA Kit

Purpose: Phosphotyrosine PD-1 ELISA Kit is a convenient and sensitive assay to detect Phosphotyrosine PD-1 in human cell lysate samples.

Analytical Method: Quantitative

Specificity: This ELISA antibody pair detects human Phospho PD-1.

Components:

• Pre-coated 96-well microplate
• Positive Control
• Anti-phospho Detection Antibody
• HRP-Streptavidin
• Wash Buffer
• Assay Diluent
• Lysis Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Material not included:

• Microplate reader capable of measuring absorbance at 450 nm
• Protease and Phosphatase inhibitors
• Shaker
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for ELISA data analysis
• Tubes to prepare the positive control or sample dilutions

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and positive control as instructed.
2. Add 100 μL of positive control and sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
3. Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
4. Add 100 μL prepared HRP-Streptavidin to each well. Incubate for 1 hour at room temperature with gentle shaking.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Target Details for PD-1

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Alternative Name: PD1

Background:

Gene Symbols: PDCD1, PD1

Protein Name (Synonyms): Programmed cell death protein 1 (Protein PD-1, hPD-1)

Gene ID: 5133

UniProt: Q15116

Pathways: Cancer Immune Checkpoints