RPS6 ELISA Kit

Catalog No. ABIN8070653

Human and Mouse RPS6 ELISA Kit, Colorimetric assay for quantification of Human and Mouse RPS6.

Quick Overview for RPS6 ELISA Kit (ABIN8070653)

Target: RPS6 (Ribosomal Protein S6 (RPS6))

Binding Specificity: pSer6, pSer240, total

Reactivity: Human, Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Cell Lysate, Tissue Lysate

Product Details RPS6 ELISA Kit

Purpose: Phospho-RPS6 (S240/244) and Total RPS6 ELISA Kit is a convenient and sensitive assay that can monitor the activation or function of important biological pathways in human, mouse cell lysates.

Analytical Method: Semi-Quantitative

Specificity: The antibody pair provided in this kit recognizes human and mouse RPS6 phosphorylated at site Serine-240/244.

Components:

• Pre-Coated 96-well Strip Microplate
• Positive Control
• Anti-Phospho Detection Antibody
• Anti-Pan Detection Antibody (*not included in phospho-only version)
• HRP-Conjugated Secondary Antibody
• Wash Buffer
• Assay Diluent
• Lysis Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Material not included:

• Microplate reader capable of measuring absorbance at 450 nm
• Protease and Phosphatase inhibitors
• Shaker
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for ELISA data analysis
• Tubes to prepare the positive control or sample dilutions

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and positive control as instructed.
2. Add 100 μL positive control or sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
3. Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
4. Add 100 μL prepared HRP-Conjugated solution. Incubate for 1 hour at room temperature with gentle shaking.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Target Details for RPS6

Target: RPS6 (Ribosomal Protein S6 (RPS6))

Alternative Name: RPS6

Background:

Gene Symbols: RPS6, OK/SW-cl.2

Protein Name (Synonyms): Small ribosomal subunit protein eS6, 40S ribosomal protein S6, Phosphoprotein NP33

Gene ID: 6194

UniProt: P62753

Pathways: Carbohydrate Homeostasis, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly