SNCA ELISA Kit

Catalog No. ABIN8070950

Human SNCA ELISA Kit, Colorimetric assay for quantification of Human SNCA.

Quick Overview for SNCA ELISA Kit (ABIN8070950)

Target: SNCA (Synuclein, alpha (SNCA))

Binding Specificity: pSer129, total

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Cell Lysate, Tissue Lysate

Product Details SNCA ELISA Kit

Purpose: Phospho-Alpha-synuclein (S129) and Total Alpha-synuclein ELISA Kit is a convenient and sensitive assay that can monitor the activation or function of important biological pathways in Human cell lysates

Analytical Method: Semi-Quantitative

Specificity: The antibody pair provided in this kit recognizes human alpha-synuclein (SNCA) phosphorylated at site Serine-129.

Components:

• Pre-Coated 96-well Strip Microplate
• Positive Control
• Anti-Phospho Detection Antibody
• Anti-Pan Detection Antibody (*not included in phospho-only version)
• HRP-Conjugated Secondary Antibody
• Wash Buffer
• Assay Diluent
• Lysis Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Material not included:

• Microplate reader capable of measuring absorbance at 450 nm
• Protease and Phosphatase inhibitors
• Shaker
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for ELISA data analysis
• Tubes to prepare the positive control or sample dilutions

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and positive control as instructed.
2. Add 100 μL positive control or sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
3. Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
4. Add 100 μL prepared HRP-Conjugated solution. Incubate for 1 hour at room temperature with gentle shaking.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Target Details for SNCA

Target: SNCA (Synuclein, alpha (SNCA))

Alternative Name: Alpha-Synuclein

Background:

Gene Symbols: SNCA, NACP, PARK1

Protein Name (Synonyms): Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor (NACP)

Gene ID: 6622

UniProt: P37840

Pathways: Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity