APAF1 CLIA Kit
Quick Overview for APAF1 CLIA Kit (ABIN8074869)
Target
See all APAF1 CLIA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 13.7 pg/mL
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Purpose
- Mouse Apoptotic Peptidase Activating Factor 1 (APAF1) CLIA Kit for detecting APAF1 in tissue homogenates
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Analytical Method
- Quantitative
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Sensitivity
- 4.87 pg/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- Substrate A
- Substrate B
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Apoptotic Peptidase Activating Factor 1 (APAF1) ELISA Kit
APAF1 Reactivity: Mouse Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Apoptotic Peptidase Activating Factor 1 (APAF1) ELISA KitAPAF1 Reactivity: Rat Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Apoptotic Peptidase Activating Factor 1 (APAF1) ELISA KitAPAF1 Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Apoptotic Peptidase Activating Factor 1 (APAF1) ELISA KitAPAF1 Reactivity: Mouse Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Cell Lysate, Tissue Homogenate
Apoptotic Peptidase Activating Factor 1 (APAF1) ELISA KitAPAF1 Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Apoptotic Peptidase Activating Factor 1 (APAF1) ELISA KitAPAF1 Reactivity: Rat Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Apoptotic Peptidase Activating Factor 1 (APAF1) ELISA KitAPAF1 Reactivity: Mouse Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 100 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))
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Alternative Name
- Apoptotic Peptidase Activating Factor 1
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Background
- Gene Aliases: CED4, Apoptotic Protease Activating Factor
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Gene ID
- 11783
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UniProt
- O88879
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Pathways
- p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity
Target See all APAF1 CLIA Kits
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