PUMA ELISA Kit

Catalog No. ABIN8075165

This Colorimetric ELISA kit is designed for the quantitative measurement of Human PUMA.

Quick Overview for PUMA ELISA Kit (ABIN8075165)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Product Details PUMA ELISA Kit

Minimum Detection Limit: 0.156 ng/mL

Purpose: Human p53 Upregulated Modulator Of Apoptosis (PUMA) ELISA Kit for detecting PUMA in tissue homogenates, cell lysates

Analytical Method: Quantitative

Sensitivity: 0.056 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details for PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Alternative Name: p53 Upregulated Modulator Of Apoptosis

Background: Gene Aliases: JFY1, BBC3, BCL2 Binding Component 3

Gene ID: 27113

UniProt: Q9BXH1

Pathways: p53 Signaling, Positive Regulation of Endopeptidase Activity