BRCA2 ELISA Kit

Catalog No. ABIN8075428

Mouse BRCA2 ELISA Kit, Colorimetric assay for quantification of Mouse BRCA2.

Quick Overview for BRCA2 ELISA Kit (ABIN8075428)

Target: BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Product Details BRCA2 ELISA Kit

Minimum Detection Limit: 0.156 ng/mL

Purpose: Mouse Breast Cancer Susceptibility Protein 2 (BRCA2) ELISA Kit for detecting BRCA2 in tissue homogenates

Analytical Method: Quantitative

Sensitivity: 0.055 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details for BRCA2

Target: BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

Alternative Name: Breast Cancer Susceptibility Protein 2

Background: Gene Aliases: BRCA2, BRCC2, FACD, FAD, FAD1, FANCD, FANCD1, Breast Cancer 2,Early Onset, Breast Cancer Type 2 Susceptibility Protein, Fanconi anemia group D1 protein

Gene ID: 12190

UniProt: P97929

Pathways: DNA Damage Repair, M Phase, Maintenance of Protein Location