Caspase 8 ELISA Kit
Quick Overview for Caspase 8 ELISA Kit (ABIN8075992)
Target
See all Caspase 8 (CASP8) ELISA KitsBinding Specificity
Reactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- Phospho-Caspase 8 (Ser347) Colorimetric Cell-Based ELISA Kit for detecting Phospho-Caspase 8 (Ser347) in researchers plate their cell line of choice.
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Analytical Method
- Qualitative
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Caspase 8 (CASP8) ELISA Kit
CASP8 Reactivity: Mouse Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Caspase 8 (CASP8) ELISA KitCASP8 Reactivity: Human Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Caspase 8 (CASP8) ELISA KitCASP8 Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Caspase 8 (CASP8) ELISA KitCASP8 Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Caspase 8 (CASP8) ELISA KitCASP8 Reactivity: Pig Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Caspase 8 (CASP8) ELISA KitCASP8 Reactivity: Dog Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Caspase 8 (CASP8) ELISA KitCASP8 Reactivity: Chicken Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Caspase 8 (CASP8) ELISA KitCASP8 Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Caspase 8 (CASP8) ELISA KitCASP8 Reactivity: Human cleaved Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Caspase 8 (CASP8) ELISA KitCASP8 Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Assay Time
- 4 - 5 h
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Plate
- Pre-coated
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 6 months
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- Caspase 8 (CASP8)
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Alternative Name
- Caspase 8
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Background
- Gene Aliases: Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, Caspase-8 precursor, FADD-homologous ICE/CED-3- like protease, FADD-like ICE, FLICE, ICE-like apoptotic protease 5, ICE8, MACH, MCH5, MORT1-associated CED-3 homolog
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Gene ID
- 841
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UniProt
- Q14790
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Pathways
- Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades
Target See all Caspase 8 (CASP8) ELISA Kits
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