CD80 ELISA Kit
Quick Overview for CD80 ELISA Kit (ABIN8076253)
Target
See all CD80 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 0.156 ng/mL
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Purpose
- Mouse B-Lymphocyte Activation Antigen B7-1 (LAB7-1) ELISA Kit for detecting LAB7-1 in tissue homogenates, cell lysates, cell culture supernates
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Analytical Method
- Quantitative
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Sensitivity
- 0.057 ng/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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CD80 (CD80) ELISA Kit
CD80 Reactivity: Human Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
CD80 (CD80) ELISA KitCD80 Reactivity: Human Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
CD80 (CD80) ELISA KitQuick CD80 Reactivity: Mouse Colorimetric Sandwich ELISA 0.39 ng/mL - 25 ng/mL Biological Fluids, Plasma, Serum
CD80 (CD80) ELISA KitCD80 Reactivity: Mouse AA 37-245 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Serum
CD80 (CD80) ELISA KitCD80 Reactivity: Mouse Colorimetric Sandwich ELISA 15.6-1000 pg/mL Plasma, Serum, Tissue Homogenate
CD80 (CD80) ELISA KitCD80 Reactivity: Rat Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
CD80 (CD80) ELISA KitCD80 Reactivity: Mouse Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
CD80 (CD80) ELISA KitCD80 Reactivity: Human Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
CD80 (CD80) ELISA KitCD80 Reactivity: Human AA 35-242 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Serum
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 100 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- CD80
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Alternative Name
- B-Lymphocyte Activation Antigen B7-1
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Background
- Gene Aliases: CD80, CD28LG, CD28LG1, BB1, B7-1 Antigen, T-lymphocyte activation antigen CD80, CTLA-4 counter-receptor B7.1
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Gene ID
- 12519
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UniProt
- Q00609
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Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
Target See all CD80 ELISA Kits
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