GM-CSF ELISA Kit

Catalog No. ABIN8077253

This Colorimetric ELISA kit is designed for the quantitative measurement of Human GM-CSF.

Quick Overview for GM-CSF ELISA Kit (ABIN8077253)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 32 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Plasma, Red Blood Cell Lysate, Serum

Product Details GM-CSF ELISA Kit

Minimum Detection Limit: 32 pg/mL

Purpose: GM-CSF (Human) Sandwich ELISA Kit for detecting GM-CSF in plasma, serum, lysate.

Analytical Method: Quantitative

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Assay Time: 4 - 5 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 6 months

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: GM-CSF

Background: Gene Aliases: Granulocyte-macrophage colony-stimulating factor, Colony-stimulating factor, CSF, Molgramostin, Sargramostim

Gene ID: 1437

UniProt: P04141

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin