C5A CLIA Kit

Catalog No. ABIN8077351

Rat C5A ELISA Kit, Chemiluminescent assay for quantification of Rat C5A.

Quick Overview for C5A CLIA Kit (ABIN8077351)

Target: C5A (C5a) (Complement Component 5a (C5a))

Reactivity: Rat

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 0.27 ng/mL - 200 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details C5A CLIA Kit

Minimum Detection Limit: 0.27 ng/mL

Purpose: Rat Complement Component 5a (C5a) CLIA Kit for detecting C5a in serum, plasma

Analytical Method: Quantitative

Sensitivity: 0.11 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• Substrate A
• Substrate B
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details

Target: C5A (C5a) (Complement Component 5a (C5a))

Alternative Name: Complement Component 5a

Background: Gene Aliases:

UniProt: P08650

Pathways: Complement System, Carbohydrate Homeostasis