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Cyclin D2 ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Cyclin D2.
Catalog No. ABIN8077718
$772.15
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 8 to 13 Business Days

Quick Overview for Cyclin D2 ELISA Kit (ABIN8077718)

Target

See all Cyclin D2 (CCND2) ELISA Kits
Cyclin D2 (CCND2)

Reactivity

  • 15
  • 8
  • 8
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

15.6 pg/mL - 1000 pg/mL

Application

ELISA

Sample Type

Cell Lysate, Tissue Homogenate
  • Minimum Detection Limit

    15.6 pg/mL

    Purpose

    Human Cyclin D2 (CCND2) ELISA Kit for detecting CCND2 in tissue homogenates, cell lysates

    Analytical Method

    Quantitative

    Sensitivity

    6.0 pg/mL

    Components

    • Pre-coated, ready to use 96-well strip plate
    • Standar
    • Detection Reagent A
    • Detection Reagent B
    • TMB Substrate
    • Stop Solution
    • Assay Diluent A
    • Assay Diluent B
    • Wash Buffer (30x concentrate)
    • Standard Diluent
    • Plate Sealer
    • Instruction Manual

    Material not included

    • Microplate Reader with 450 nm filter
    • Single or multi-channel pipettes with high precision and disposable tips
    • Microcentrifuge tubes
    • Deionized or distilled watter
    • Absorbent paper for blotting the microplate
    • Container for wash solution
    • 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

    Sample Volume

    100 μL

    Assay Time

    3 h

    Plate

    Pre-coated

    Assay Precision

    Intra-Assay: CV<10%,
    Inter-Assay: CV<12%

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    4°C for one month, Long term: see manual.

    Expiry Date

    12 months
  • Target See all Cyclin D2 (CCND2) ELISA Kits

    Cyclin D2 (CCND2)

    Alternative Name

    Cyclin D2

    Background

    Gene Aliases: CCN-D2, G1/S-Specific Cyclin-D2

    Gene ID

    894

    UniProt

    P30279

    Pathways

    Cell Division Cycle, Mitotic G1-G1/S Phases
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