EGFR ELISA Kit

Catalog No. ABIN8078621

This Colorimetric ELISA kit is designed for the quantitative measurement of Human EGFR.

Quick Overview for EGFR ELISA Kit (ABIN8078621)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 3.12 ng/mL - 200 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details EGFR ELISA Kit

Minimum Detection Limit: 3.12 ng/mL

Purpose: Human Epidermal Growth Factor Receptor (EGFR) Anti-antigen ELISA Kit for detecting EGFR in serum, plasma

Analytical Method: Quantitative

Sensitivity: 1.17 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 2.5 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: Epidermal Growth Factor Receptor

Background: Gene Aliases: ErbB-1, ErbB1, ER1, ERB-B1, MENA, HER1, Erythroblastic Leukemia Viral (v-Erb-B) oncogene Homolog,Avian, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

Gene ID: 1956

UniProt: P00533

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins