GRB2 ELISA Kit

Catalog No. ABIN8080241

This Colorimetric ELISA kit is designed for the quantitative measurement of Human, Mouse and Rat GRB2.

Quick Overview for GRB2 ELISA Kit (ABIN8080241)

Target: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Binding Specificity: pSer159

Reactivity: Human, Mouse, Rat

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Culture Cells

Product Details GRB2 ELISA Kit

Purpose: Phospho-GRB2 (Ser159) Colorimetric Cell-Based ELISA Kit for detecting Phospho-GRB2 (Ser159) in researchers plate their cell line of choice.

Analytical Method: Qualitative

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Assay Time: 4 - 5 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 6 months

Target Details for GRB2

Target: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Alternative Name: GRB2

Background: Gene Aliases: GRB2-associated-binding protein 2, Growth factor receptor bound protein 2-associated protein 2, GRB2-associated binder 2, pp100, KIAA0571

Gene ID: 9846

UniProt: Q9UQC2

Pathways: RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation