HSP27 ELISA Kit
Quick Overview for HSP27 ELISA Kit (ABIN8080352)
Target
See all HSP27 (HSPB1) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- HSP27 Colorimetric Cell-Based ELISA Kit for detecting HSP27 in researchers plate their cell line of choice.
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Analytical Method
- Qualitative
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Heat Shock 27kDa Protein 1 (HSPB1) ELISA Kit
HSPB1 Reactivity: Rat Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Heat Shock 27kDa Protein 1 (HSPB1) ELISA KitHSPB1 Reactivity: Human AA 2-205 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Heat Shock 27kDa Protein 1 (HSPB1) ELISA KitHSPB1 Reactivity: Rat Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Heat Shock 27kDa Protein 1 (HSPB1) ELISA KitHSPB1 Reactivity: Mouse Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Heat Shock 27kDa Protein 1 (HSPB1) ELISA KitHSPB1 Reactivity: Cow Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Heat Shock 27kDa Protein 1 (HSPB1) ELISA KitHSPB1 Reactivity: Human Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Heat Shock 27kDa Protein 1 (HSPB1) ELISA KitHSPB1 Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Heat Shock 27kDa Protein 1 (HSPB1) ELISA KitHSPB1 Reactivity: Human Colorimetric Sandwich ELISA 781 pg/mL - 50000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Heat Shock 27kDa Protein 1 (HSPB1) ELISA KitHSPB1 Reactivity: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Assay Time
- 4 - 5 h
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Plate
- Pre-coated
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 6 months
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- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
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Alternative Name
- HSP27
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Background
- Gene Aliases: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Growth-related 25 kDa protein, Heat shock 27 kDa protein, HSP 27, HSP25, HSPB1, P25, SRP27, Stress-responsive protein 27
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Gene ID
- 3315
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UniProt
- P04792
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Pathways
- MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
Target See all HSP27 (HSPB1) ELISA Kits
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