HNF4A ELISA Kit

Catalog No. ABIN8080563

The Human, Mouse and Rat HNF4A ELISA Kit (ABIN8080563) is a Colorimetric ELISA Kit designed to quantify Human, Mouse and Rat HNF4A.

Quick Overview for HNF4A ELISA Kit (ABIN8080563)

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Binding Specificity: pSer313

Reactivity: Human, Mouse, Rat

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Culture Cells

Product Details HNF4A ELISA Kit

Purpose: Phospho-HNF4α (Ser313) Colorimetric Cell-Based ELISA Kit for detecting Phospho-HNF4α (Ser313) in researchers plate their cell line of choice.

Analytical Method: Qualitative

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Assay Time: 4 - 5 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 6 months

Target Details for HNF4A

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Alternative Name: HNF4alpha

Background: Gene Aliases: HNF-4, HNF-4-alpha, HNF4, HNF4A, Hepatocyte nuclear factor 4-alpha, NR2A1, TCF14, Transcription factor 14, Transcription factor HNF-4

Gene ID: 3172

UniProt: P41235

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process