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Hexokinase 1 ELISA Kit

Mouse Hexokinase 1 ELISA Kit, Colorimetric assay for quantification of Mouse Hexokinase 1.
Catalog No. ABIN8080600
$1,021.38
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 8 to 13 Business Days

Quick Overview for Hexokinase 1 ELISA Kit (ABIN8080600)

Target

See all Hexokinase 1 (HK1) ELISA Kits
Hexokinase 1 (HK1)

Reactivity

  • 12
  • 6
  • 5
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Mouse

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

62.5 pg/mL - 4000 pg/mL

Application

ELISA

Sample Type

Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
  • Minimum Detection Limit

    62.5 pg/mL

    Purpose

    Mouse Hexokinase 1 (Hk1) ELISA Kit for detecting HK1 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates

    Analytical Method

    Quantitative

    Sensitivity

    28.7 pg/mL

    Components

    • Pre-coated, ready to use 96-well strip plate
    • Standar
    • Detection Reagent A
    • Detection Reagent B
    • TMB Substrate
    • Stop Solution
    • Assay Diluent A
    • Assay Diluent B
    • Wash Buffer (30x concentrate)
    • Standard Diluent
    • Plate Sealer
    • Instruction Manual

    Material not included

    • Microplate Reader with 450 nm filter
    • Single or multi-channel pipettes with high precision and disposable tips
    • Microcentrifuge tubes
    • Deionized or distilled watter
    • Absorbent paper for blotting the microplate
    • Container for wash solution
    • 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

    Sample Volume

    100 μL

    Assay Time

    3 h

    Plate

    Pre-coated

    Assay Precision

    Intra-Assay: CV<10%,
    Inter-Assay: CV<12%

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    4°C for one month, Long term: see manual.

    Expiry Date

    12 months
  • Target See all Hexokinase 1 (HK1) ELISA Kits

    Hexokinase 1 (HK1)

    Alternative Name

    Hexokinase 1

    Background

    Gene Aliases: HK1-Ta, HK1-Tb, HK1-Tc, HKI, HXK1, Brain form hexokinase

    Gene ID

    15275

    UniProt

    P17710

    Pathways

    Carbohydrate Homeostasis, Warburg Effect
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