IRS1 ELISA Kit
Quick Overview for IRS1 ELISA Kit (ABIN8081175)
Target
See all IRS1 ELISA KitsBinding Specificity
Reactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- Phospho-IRS-1 (Ser636) Colorimetric Cell-Based ELISA Kit for detecting Phospho-IRS-1 (Ser636) in researchers plate their cell line of choice.
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Analytical Method
- Qualitative
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Insulin Receptor Substrate 1 (IRS1) ELISA Kit
IRS1 Reactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin Receptor Substrate 1 (IRS1) ELISA KitIRS1 Reactivity: Human Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Insulin Receptor Substrate 1 (IRS1) ELISA KitIRS1 Reactivity: Rat Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Insulin Receptor Substrate 1 (IRS1) ELISA KitIRS1 Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Insulin Receptor Substrate 1 (IRS1) ELISA KitIRS1 Reactivity: Mouse Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin Receptor Substrate 1 (IRS1) ELISA KitIRS1 Reactivity: Human Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Insulin Receptor Substrate 1 (IRS1) ELISA KitIRS1 Reactivity: Mouse Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin Receptor Substrate 1 (IRS1) ELISA KitIRS1 Reactivity: Human Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Insulin Receptor Substrate 1 (IRS1) ELISA KitIRS1 Reactivity: Rat Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Assay Time
- 4 - 5 h
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Plate
- Pre-coated
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 6 months
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- IRS1 (Insulin Receptor Substrate 1 (IRS1))
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Alternative Name
- IRS-1
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Background
- Gene Aliases: Insulin receptor substrate-1, IRS-1, IRS1
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Gene ID
- 3667
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UniProt
- P35568
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Pathways
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process
Target See all IRS1 ELISA Kits
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