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IRF3 ELISA Kit

Human IRF3 ELISA Kit, Colorimetric assay for quantification of Human IRF3.
Catalog No. ABIN8081497
$806.08
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 8 to 13 Business Days

Quick Overview for IRF3 ELISA Kit (ABIN8081497)

Target

See all IRF3 ELISA Kits
IRF3 (Interferon Regulatory Factor 3 (IRF3))

Reactivity

  • 22
  • 11
  • 3
  • 2
  • 1
  • 1
Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

0.156 ng/mL - 10 ng/mL

Application

ELISA

Sample Type

Cell Lysate, Tissue Homogenate
  • Minimum Detection Limit

    0.156 ng/mL

    Purpose

    Human Interferon Regulatory Factor 3 (IRF3) ELISA Kit for detecting IRF3 in tissue homogenates, cell lysates

    Analytical Method

    Quantitative

    Sensitivity

    0.055 ng/mL

    Components

    • Pre-coated, ready to use 96-well strip plate
    • Standar
    • Detection Reagent A
    • Detection Reagent B
    • TMB Substrate
    • Stop Solution
    • Assay Diluent A
    • Assay Diluent B
    • Wash Buffer (30x concentrate)
    • Standard Diluent
    • Plate Sealer
    • Instruction Manual

    Material not included

    • Microplate Reader with 450 nm filter
    • Single or multi-channel pipettes with high precision and disposable tips
    • Microcentrifuge tubes
    • Deionized or distilled watter
    • Absorbent paper for blotting the microplate
    • Container for wash solution
    • 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

    Sample Volume

    100 μL

    Assay Time

    3 h

    Plate

    Pre-coated

    Assay Precision

    Intra-Assay: CV<10%,
    Inter-Assay: CV<12%

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    4°C for one month, Long term: see manual.

    Expiry Date

    12 months
  • Target See all IRF3 ELISA Kits

    IRF3 (Interferon Regulatory Factor 3 (IRF3))

    Alternative Name

    Interferon Regulatory Factor 3

    Background

    Gene Aliases:

    Gene ID

    3661

    UniProt

    Q14653

    Pathways

    TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades
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