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MEP1A ELISA Kit

Human MEP1A ELISA Kit, Colorimetric assay for quantification of Human MEP1A.
Catalog No. ABIN8083468
$772.15
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 8 to 13 Business Days

Quick Overview for MEP1A ELISA Kit (ABIN8083468)

Target

See all MEP1A ELISA Kits
MEP1A (Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A))

Reactivity

  • 12
  • 8
  • 7
Human

Detection Method

Colorimetric

Method Type

Competition ELISA

Detection Range

49.4 pg/mL - 4000 pg/mL

Application

ELISA

Sample Type

Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
  • Minimum Detection Limit

    49.4 pg/mL

    Purpose

    Human Meprin A Alpha (MEP1a) ELISA Kit for detecting MEP1a in serum, plasma, tissue homogenates, cell lysates, cell culture supernates

    Analytical Method

    Quantitative

    Sensitivity

    19.4 pg/mL

    Components

    • Pre-coated, ready to use 96-well strip plate
    • Standar
    • Detection Reagent A
    • Detection Reagent B
    • TMB Substrate
    • Stop Solution
    • Assay Diluent A
    • Assay Diluent B
    • Wash Buffer (30x concentrate)
    • Standard Diluent
    • Plate Sealer
    • Instruction Manual

    Material not included

    • Microplate Reader with 450 nm filter
    • Single or multi-channel pipettes with high precision and disposable tips
    • Microcentrifuge tubes
    • Deionized or distilled watter
    • Absorbent paper for blotting the microplate
    • Container for wash solution
    • 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

    Sample Volume

    50 μL

    Assay Time

    2 h

    Plate

    Pre-coated

    Assay Precision

    Intra-Assay: CV<10%,
    Inter-Assay: CV<12%

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    4°C for one month, Long term: see manual.

    Expiry Date

    12 months
  • Target See all MEP1A ELISA Kits

    MEP1A (Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A))

    Alternative Name

    Meprin A Alpha

    Background

    Gene Aliases: PPHA, PABA Peptide Hydrolase, Endopeptidase-2, N-benzoyl-L-tyrosyl-P-amino-Benzoic Acid Hydrolase Subunit Alpha, PABA Peptide Hydrolase

    Gene ID

    4224

    UniProt

    Q16819
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