Midkine ELISA Kit
Quick Overview for Midkine ELISA Kit (ABIN8083619)
Target
See all Midkine (MDK) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 32 pg/mL
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Purpose
- Midkine (Human) Sandwich ELISA Kit for detecting Midkine in plasma, serum, lysate.
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Analytical Method
- Quantitative
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Midkine (Neurite Growth-Promoting Factor 2) (MDK) ELISA Kit
MDK Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Midkine (Neurite Growth-Promoting Factor 2) (MDK) ELISA KitMDK Reactivity: Human Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Midkine (Neurite Growth-Promoting Factor 2) (MDK) ELISA KitMDK Reactivity: Mouse Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Midkine (Neurite Growth-Promoting Factor 2) (MDK) ELISA KitMDK Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Midkine (Neurite Growth-Promoting Factor 2) (MDK) ELISA KitMDK Reactivity: Human Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Midkine (Neurite Growth-Promoting Factor 2) (MDK) ELISA KitMDK Reactivity: Rat AA 21-140 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Midkine (Neurite Growth-Promoting Factor 2) (MDK) ELISA KitMDK Reactivity: Mouse AA 23-140 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Midkine (Neurite Growth-Promoting Factor 2) (MDK) ELISA KitMDK Reactivity: Human Colorimetric Sandwich ELISA 32-2000 pg/mL Cell Lysate, Plasma, Serum
Midkine (Neurite Growth-Promoting Factor 2) (MDK) ELISA KitMDK Reactivity: Human Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Plasma, Serum
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Assay Time
- 4 - 5 h
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Plate
- Pre-coated
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 6 months
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- Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))
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Alternative Name
- Midkine
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Background
- Gene Aliases: MK, Amphiregulin-associated protein, ARAP, Midgestation and kidney protein, Neurite outgrowth-promoting factor 2, Neurite outgrowth-promoting protein, MK1, NEGF2
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Gene ID
- 4192
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UniProt
- P21741
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Pathways
- RTK Signaling, M Phase, Skeletal Muscle Fiber Development
Target See all Midkine (MDK) ELISA Kits
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