MAPK6 ELISA Kit

Catalog No. ABIN8083691

This Colorimetric ELISA kit is designed for the quantitative measurement of Human, Mouse and Rat MAPK6.

Quick Overview for MAPK6 ELISA Kit (ABIN8083691)

Target: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Binding Specificity: pSer189

Reactivity: Human, Mouse, Rat

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Culture Cells

Product Details MAPK6 ELISA Kit

Purpose: Phospho-ERK3 (Ser189) Colorimetric Cell-Based ELISA Kit for detecting Phospho-ERK3 (Ser189) in researchers plate their cell line of choice.

Analytical Method: Qualitative

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Assay Time: 4 - 5 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 6 months

Target Details for MAPK6

Target: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Alternative Name: ERK3

Background: Gene Aliases: ERK-3, ERK3, Extracellular signal- regulated kinase 3, MAP kinase isoform p97, MAPK6, MK06, Mitogen-activated protein kinase 6, PRKM4, PRKM6, kinase ERK3, p55-MAPK, p97-MAPK

Gene ID: 5597

UniProt: Q16659

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C