NOD2 CLIA Kit

Catalog No. ABIN8084614

Human NOD2 ELISA Kit, Chemiluminescent assay for quantification of Human NOD2.

Quick Overview for NOD2 CLIA Kit (ABIN8084614)

Target: NOD2 (Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 41.2 pg/mL - 30000 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Product Details NOD2 CLIA Kit

Minimum Detection Limit: 41.2 pg/mL

Purpose: Human Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2) CLIA Kit for detecting NOD2 in tissue homogenates, cell lysates

Analytical Method: Quantitative

Sensitivity: 17.4 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• Substrate A
• Substrate B
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details

Target: NOD2 (Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2))

Alternative Name: Nucleotide Binding Oligomerization Domain Containing Protein 2

Background: Gene Aliases: CD, ACUG, BLAU, CARD15, CLR16.3, IBD1, NLRC2, NOD2B, PSORAS1, Caspase recruitment domain-containing protein 15, Inflammatory bowel disease protein 1

Gene ID: 64127

UniProt: Q9HC29

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome