MGMT ELISA Kit

Catalog No. ABIN8084621

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat MGMT.

Quick Overview for MGMT ELISA Kit (ABIN8084621)

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.12 pg/mL - 5000 pg/mL

Application: ELISA

Sample Type: Tissue Homogenate

Product Details MGMT ELISA Kit

Minimum Detection Limit: 78.12 pg/mL

Purpose: Rat O-6-Methylguanine DNA Methyltransferase (MGMT) ELISA Kit for detecting MGMT in tissue homogenates

Analytical Method: Quantitative

Sensitivity: 27 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details for MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Alternative Name: O-6-Methylguanine DNA Methyltransferase

Background: Gene Aliases: Methylated-DNA--Protein-Cysteine Methyltransferase, 6-O-methylguanine-DNA methyltransferase, Methylated-DNA--protein-cysteine methyltransferase

Gene ID: 25332

UniProt: P24528

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus