PAK1/2/3 ELISA Kit

Catalog No. ABIN8084785

Human, Mouse and Rat PAK1/2/3 ELISA Kit, Colorimetric assay for quantification of Human, Mouse and Rat PAK1/2/3.

Quick Overview for PAK1/2/3 ELISA Kit (ABIN8084785)

Target: PAK1/2/3 (P21-Activated Kinases 1/2/3 (PAK1/2/3))

Binding Specificity: pSer139, pSer141, pSer144

Reactivity: Human, Mouse, Rat

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Culture Cells

Product Details PAK1/2/3 ELISA Kit

Purpose: Phospho-PAK1/2/3 (Ser144/141/139) Colorimetric Cell-Based ELISA Kit for detecting Phospho-PAK1/2/3 (Ser144/141/139) in researchers plate their cell line of choice.

Analytical Method: Qualitative

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Assay Time: 4 - 5 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 6 months

Target Details for PAK1/2/3

Target: PAK1/2/3 (P21-Activated Kinases 1/2/3 (PAK1/2/3))

Alternative Name: PAK1/2/3

Background: Gene Aliases: Gamma-PAK, P21-activated kinase 2, PAK 2, PAK-2, PAK65, PAKI, S6/H4 kinase, kinase PAK2, p21-activated kinase 2, p21-activated protein kinase IBeta-PAK, CDC42/RAC effector kinase PAK-B, OPHN3, Oligophrenin-3, P65-PAK, PAK 3, PAK-3, PAKB, STK4, Serine/threonine-protein kinase PAK 3, kinase PAK3, p21-activated kinase 3

Gene ID: 5058, 5062

UniProt: Q13153