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PDGFB CLIA Kit

This Chemiluminescent ELISA kit is designed for the quantitative measurement of Rat PDGFB.
Catalog No. ABIN8085323
$1,045.50
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 8 to 13 Business Days

Quick Overview for PDGFB CLIA Kit (ABIN8085323)

Target

See all PDGFB CLIA Kits
PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))

Reactivity

  • 18
  • 14
  • 12
  • 10
  • 6
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Rat

Detection Method

Chemiluminescent

Method Type

Sandwich ELISA

Detection Range

2.74 pg/mL - 2000 pg/mL

Application

ELISA

Sample Type

Plasma
  • Minimum Detection Limit

    2.74 pg/mL

    Purpose

    Rat Platelet Derived Growth Factor Subunit B (PDGFB) CLIA Kit for detecting PDGFB in plasma.

    Analytical Method

    Quantitative

    Sensitivity

    1.24 pg/mL

    Components

    • Pre-coated, ready to use 96-well strip plate
    • Standar
    • Detection Reagent A
    • Detection Reagent B
    • Substrate A
    • Substrate B
    • Assay Diluent A
    • Assay Diluent B
    • Wash Buffer (30x concentrate)
    • Standard Diluent
    • Plate Sealer
    • Instruction Manual

    Material not included

    • Microplate Reader with 450 nm filter
    • Single or multi-channel pipettes with high precision and disposable tips
    • Microcentrifuge tubes
    • Deionized or distilled watter
    • Absorbent paper for blotting the microplate
    • Container for wash solution
    • 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

    Sample Volume

    100 μL

    Assay Time

    3 h

    Plate

    Pre-coated

    Assay Precision

    Intra-Assay: CV<10%,
    Inter-Assay: CV<12%

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    4°C for one month, Long term: see manual.

    Expiry Date

    12 months
  • Target See all PDGFB CLIA Kits

    PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))

    Alternative Name

    Platelet Derived Growth Factor Subunit B

    Background

    Gene Aliases: PDGF-B, PDGF2, SIS, SSV, C-sis, Simian Sarcoma Viral (v-sis) Oncogene Homolog, Platelet Derived Growth Factor Beta Polypeptide, Proto-oncogene c-Sis, Becaplermin

    Gene ID

    24628

    UniProt

    Q05028

    Pathways

    RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
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