PKC delta ELISA Kit
Quick Overview for PKC delta ELISA Kit (ABIN8085857)
Target
See all PKC delta (PKCd) ELISA KitsBinding Specificity
Reactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- Phospho-PKC δ (Tyr313) Colorimetric Cell-Based ELISA Kit for detecting Phospho-PKC δ (Tyr313) in researchers plate their cell line of choice.
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Analytical Method
- Qualitative
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Protein Kinase C, delta (PKCd) ELISA Kit
PKCd Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Protein Kinase C, delta (PKCd) ELISA KitPKCd Reactivity: Mouse Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, delta (PKCd) ELISA KitPKCd Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Protein Kinase C, delta (PKCd) ELISA KitPKCd Reactivity: Rat Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, delta (PKCd) ELISA KitPKCd Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Protein Kinase C, delta (PKCd) ELISA KitPKCd Reactivity: Rat Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Protein Kinase C, delta (PKCd) ELISA KitPKCd Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Assay Time
- 4 - 5 h
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Plate
- Pre-coated
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 6 months
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- PKC delta (PKCd) (Protein Kinase C, delta (PKCd))
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Alternative Name
- PKC delta
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Background
- Gene Aliases: KPCD, PKC-delta, PRKCD, kinase PKC-delta, nPKC-delta
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Gene ID
- 5580
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UniProt
- Q05655
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Pathways
- Interferon-gamma Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, Lipid Metabolism
Target See all PKC delta (PKCd) ELISA Kits
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