PPP2CA ELISA Kit

Catalog No. ABIN8085922

Human PPP2CA ELISA Kit, Colorimetric assay for quantification of Human PPP2CA.

Quick Overview for PPP2CA ELISA Kit (ABIN8085922)

Target: PPP2CA (Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78 pg/mL - 5000 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Product Details PPP2CA ELISA Kit

Minimum Detection Limit: 78 pg/mL

Purpose: Human Protein Phosphatase 2, Catalytic Subunit Alpha Isoform (PPP2Ca) ELISA Kit for detecting PPP2Ca in tissue homogenates, cell lysates

Analytical Method: Quantitative

Sensitivity: 30 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details for PPP2CA

Target: PPP2CA (Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA))

Alternative Name: Protein Phosphatase 2, Catalytic Subunit Alpha Isoform

Background: Gene Aliases: PP2CA, PP2Ac, RP-C, Replication protein C, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

Gene ID: 5515

UniProt: P67775

Pathways: PI3K-Akt Signaling, Mitotic G1-G1/S Phases, Hepatitis C, Toll-Like Receptors Cascades