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SFRP5 ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Human SFRP5.
Catalog No. ABIN8086616
$840.00
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 8 to 13 Business Days

Quick Overview for SFRP5 ELISA Kit (ABIN8086616)

Target

See all SFRP5 ELISA Kits
SFRP5 (Secreted Frizzled-Related Protein 5 (SFRP5))

Reactivity

  • 20
  • 8
  • 7
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

1.56 ng/mL - 100 ng/mL

Application

ELISA

Sample Type

Plasma, Serum, Tissue Homogenate
  • Minimum Detection Limit

    1.56 ng/mL

    Purpose

    Human Secreted Frizzled Related Protein 5 (SFRP5) ELISA Kit for detecting SFRP5 in serum, plasma, tissue homogenates

    Analytical Method

    Quantitative

    Sensitivity

    0.58 ng/mL

    Components

    • Pre-coated, ready to use 96-well strip plate
    • Standar
    • Detection Reagent A
    • Detection Reagent B
    • TMB Substrate
    • Stop Solution
    • Assay Diluent A
    • Assay Diluent B
    • Wash Buffer (30x concentrate)
    • Standard Diluent
    • Plate Sealer
    • Instruction Manual

    Material not included

    • Microplate Reader with 450 nm filter
    • Single or multi-channel pipettes with high precision and disposable tips
    • Microcentrifuge tubes
    • Deionized or distilled watter
    • Absorbent paper for blotting the microplate
    • Container for wash solution
    • 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

    Sample Volume

    100 μL

    Assay Time

    3 h

    Plate

    Pre-coated

    Assay Precision

    Intra-Assay: CV<10%,
    Inter-Assay: CV<12%

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    4°C for one month, Long term: see manual.

    Expiry Date

    12 months
  • Target See all SFRP5 ELISA Kits

    SFRP5 (Secreted Frizzled-Related Protein 5 (SFRP5))

    Alternative Name

    Secreted Frizzled Related Protein 5

    Background

    Gene Aliases: SARP3, Secreted Apoptosis Related Protein 3, Frizzled-related protein 1b

    Gene ID

    6425

    UniProt

    Q5T4F7

    Pathways

    WNT Signaling
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