THRA ELISA Kit

Catalog No. ABIN8087896

Human THRA ELISA Kit, Colorimetric assay for quantification of Human THRA.

Quick Overview for THRA ELISA Kit (ABIN8087896)

Target: THRA (Thyroid Hormone Receptor, alpha (THRA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Application: ELISA

Sample Type: Tissue Homogenate

Product Details THRA ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Purpose: Human Thyroid Hormone Receptor Alpha (THRa) ELISA Kit for detecting THRa in tissue homogenates

Analytical Method: Quantitative

Sensitivity: 22.5 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Material not included:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Assay Precision: Intra-Assay: CV<10%,
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C for one month, Long term: see manual.

Expiry Date: 12 months

Target Details for THRA

Target: THRA (Thyroid Hormone Receptor, alpha (THRA))

Alternative Name: Thyroid Hormone Receptor Alpha

Background: Gene Aliases: THR-A, NR1A1, AR7, EAR7, ERB-T1, ERBA1, NR1-A1, THRA1, THRA2, c-ERBA1, Erythroblastic Leukemia Viral Oncogene Homolog, Nuclear Receptor Subfamily 1,Group A,Member 1

Gene ID: 7067

UniProt: P10827

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Sensory Perception of Sound, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Maintenance of Protein Location, Skeletal Muscle Fiber Development