Growth Hormone 1 ELISA Kit

Catalog No. ABIN8118913

The Human Growth Hormone 1 ELISA Kit (ABIN8118913) is a Colorimetric ELISA Kit designed to quantify Human Growth Hormone 1.

Quick Overview for Growth Hormone 1 ELISA Kit (ABIN8118913)

Target: Growth Hormone 1 (GH1)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 9.375 ng/mL - 500 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details Growth Hormone 1 ELISA Kit

Minimum Detection Limit: 9.375 ng/mL

Purpose: Quantification of human growth hormone in biological matrices

Analytical Method: Quantitative

Specificity: HGH

Components:

• Coated microtiter plate, 96 wells
• (1x8 strips) 1 Calibrator diluent 1.8ml
• Calibrator (1mg/ml) 12μl 20X wash buffer 25ml
• Assay buffer 50ml
• 1000X secondary antibody 1000X detection reagent 17μl TMB 12ml
• TMB stop solution 12ml

Material not included:

• Precision pipettes calibrated to deliver 5-1000μL
• Multi-channel pipette calibrated to deliver 50-200μL
• Plate shaker
• Disposable tips
• Vortex-Mixe
• Distilled or de-ionized water
• Microplate reader capable of reading 450nm with background subtraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 15 μL

Assay Time: 2.5 h

Plate: Pre-coated

Protocol: This assay employs the indirect sandwich enzyme immunoassay technique. Anti-GH is coated onto a 96 well microplate. Calibrator and test samples prepared by dilution into assay buffer and are pipetted into the appropriate wells. GH present in biological matrices is bound by the immobilized anti- GH antibody. After washing away any unbound substances, tagged antiGH secondary antibody is added to the wells. After washing, diluted detection reagent is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of GH present in test samples. The color development is stopped and the intensity of the color is measured.

Reagent Preparation:

Prepare only the appropriate amount of required reagent on the day of use. Store all reagents as per instructions stated on the label. 1. Wash Buffer (1X) Preparation: Dilute wash buffer concentrate with ultra-pure water 1/20 before use (for example add 25 mL concentrate to 475 mL ultrapure water). Mix well. 2. Secondary Antibody (1X) Preparation: Dilute secondary antibody with assay buffer 1/1000 before use (for example add 12 μL to 12 mL of assay buffer). Mix well. 3. Detection Reagent (1X) Preparation: Dilute detection reagent with assay buffer 1/1000 before use (for example add 12 μL concentrate to 12 mL of assay buffer). Mix well. 4. Calibrator Preparation: Dilute the calibrator from 1 mg/mL down to 5 μg/mL by pipetting 5μL of calibrator stock into 995μL assay buffer. Label "Cal. Int." Mix well. Prepare calibrators with concentrations ranging from 500 ng/mL to 9.375 ng/mL. The following is an example calibrator curve.

Sample Collection: This kit is compatible with EDTA-plasma, heparinplasma and serum samples. Samples can be stored at or below -20 °C for up to 1 year.

Sample Preparation:

Dilute calibrators and test samples 1/50 with assay buffer (for example add 5μL of prepared calibrator or sample to 245μL of assay buffer). Mix well. Do not store diluted samples.

Calculation of Results:

1. Construct a standard curve by plotting the absorbance obtained from each standard against concentration. Use a 4 or 5 parameter curve fit. 2. The concentration of the unknowns can be back calculated directly from this standard curve using the absorbance value for each sample. 3. Any sample diluted more or less than the standard series will need additional data correction. For example, if the sample is diluted 1/50, then the concentration will be calculated by dividing by 2 due to the calibrators being 2 times more diluted. Similarly, if the sample is diluted 1/500, then the concentration will be calculated by multiplying by a correction factor of 5 due to the calibrators being 5 times more concentrated.

Assay Precision: < 25% for ULOQ and LLOQ and <20% for the remaining concentrations

Restrictions: For Research Use only

Handling

Preservative: Without preservative

Storage: -20 °C

Storage Comment: -20C, 1 year

Expiry Date: 12 months

Target Details for Growth Hormone 1

Target: Growth Hormone 1 (GH1)

Alternative Name: growth hormone

Background: Growth hormone (GH) or somatotropin, also known as human growth hormone (hGH or HGH) in its human form, is a peptide hormone that stimulates growth, cell reproduction, and cell regeneration in humans and other animals. It is thus important in human development. GH also stimulates production of IGF-1 and increases the concentration of glucose and free fatty acids

Gene ID: 2688

Pathways: NF-kappaB Signaling, JAK-STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process