GM-CSF ELISA Kit

Catalog No. ABIN8126236

The Human GM-CSF ELISA Kit (ABIN8126236) is a Colorimetric ELISA Kit designed to quantify Human GM-CSF.

Quick Overview for GM-CSF ELISA Kit (ABIN8126236)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.9 pg/mL - 250 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details GM-CSF ELISA Kit

Minimum Detection Limit: 3.9 pg/mL

Purpose: This kit was based on sandwich enzyme-linked immune-sorbent assay technology.

Analytical Method: Quantitative

Sensitivity: 2.34 pg/mL

Characteristics: This immunoassay kit allows for the in vitro quantitative determination of Human GMCSF concentrations in serum, plasma. Detection Range: 3.9 - 250 pg/mL Sensitivity: < 2.34 pg/mL

Application Details

Plate: Pre-coated

Protocol: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human GMCSF antibody was pre-coated into 96-well plates. Biotin conjugated anti-human GMCSF detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human GMCSF amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human GMCSF can be calculated using the standard curve.

Restrictions: For Research Use only

Handling

Storage Comment: Please refer to the Manual

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: GMCSF (Granulocyte Macrophage Colony Stimulating Factor)

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin