VEGFA ELISA Kit

Catalog No. ABIN8126240

Human VEGFA ELISA Kit, Colorimetric assay for quantification of Human VEGFA.

Quick Overview for VEGFA ELISA Kit (ABIN8126240)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details VEGFA ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: This assay employs an antibody specific for Anti-human VEGF coated on a 96-well plate.

Analytical Method: Quantitative

Sensitivity: 9.0 pg/mL

Characteristics: This immunoassay kit allows for the in vitro quantitative determination of Human VEGF concentrations in serum, plasma.  Detection Range: 31.2 - 2000 pg/mL Sensitivity: < 9.0 pg/mL

Application Details

Plate: Pre-coated

Protocol: This assay employs an antibody specific for Anti-human VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.    

Restrictions: For Research Use only

Handling

Storage Comment: Please refer to the Manual

Target Details for VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name: VEGF (Vascular Endothelial Cell Growth Factor)

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling