IL-13 ELISA Kit
Quick Overview for IL-13 ELISA Kit (ABIN8126245)
Target
See all IL-13 (IL13) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 31.25 pg/mL
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Purpose
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology.
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Analytical Method
- Quantitative
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Sensitivity
- 18.75 pg/mL
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Characteristics
- This immunoassay kit allows for the in vitro quantitative determination of Mouse IL-13 concentrations in serum, plasma. Detection Range: 31.25 - 2000 pg/mL Sensitivity: < 18.75 pg/mL
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Interleukin 13 (IL13) ELISA Kit
IL13 Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 13 (IL13) ELISA KitIL13 Reactivity: Rat Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 13 (IL13) ELISA KitIL13 Reactivity: Pig Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 13 (IL13) ELISA KitIL13 Reactivity: Dog Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 13 (IL13) ELISA KitIL13 Reactivity: Mouse Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 13 (IL13) ELISA KitIL13 Reactivity: Chicken Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 13 (IL13) ELISA KitIL13 Reactivity: Cow Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 13 (IL13) ELISA KitIL13 Reactivity: Guinea Pig Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 13 (IL13) ELISA KitIL13 Reactivity: Human Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 13 (IL13) ELISA KitIL13 Reactivity: Mouse Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
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Plate
- Pre-coated
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Protocol
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL-13 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-13 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-13 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader, and then the concentration of IL-13 can be calculated.
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Restrictions
- For Research Use only
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Storage Comment
- Please refer to the Manual
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- IL-13 (IL13) (Interleukin 13 (IL13))
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Alternative Name
- IL-13 (Interleukin 13)
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Pathways
- JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport
Target See all IL-13 (IL13) ELISA Kits
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