IL-21 ELISA Kit

Catalog No. ABIN8126247

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse IL-21.

Quick Overview for IL-21 ELISA Kit (ABIN8126247)

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details IL-21 ELISA Kit

Minimum Detection Limit: 31.25 pg/mL

Purpose: This assay employs an antibody specific for Anti-Mouse IL-21 coated on a 96-well plate.

Analytical Method: Quantitative

Characteristics: This immunoassay kit allows for the in vitro quantitative determination of Mouse IL-21 concentrations in serum, plasma.  Detection Range: 31.25 - 2000 pg/mL Sensitivity: <18.75pg / ml    

Application Details

Plate: Pre-coated

Protocol: This assay employs an antibody specific for Anti-Mouse IL-21 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-21 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-21 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-21 bound.The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. 

Restrictions: For Research Use only

Handling

Storage Comment: Please refer to the Manual

Target Details for IL-21

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Alternative Name: IL-21 (Interleukin 21)

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response