IL-4 ELISA Kit

Catalog No. ABIN8126250

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse IL-4.

Quick Overview for IL-4 ELISA Kit (ABIN8126250)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.625 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details IL-4 ELISA Kit

Minimum Detection Limit: 15.625 pg/mL

Purpose: This assay employs an antibody specific for Anti-mouse IL-4 coated on a 96-well plate.

Analytical Method: Quantitative

Characteristics: This immunoassay kit allows for the in vitro quantitative determination of Mouse IL-4 concentrations in serum, plasma.  Detection Range: 15.625-1000pg/mL Sensitivity: <9.375pg/mL  

Application Details

Plate: Pre-coated

Protocol: This assay employs an antibody specific for Anti-mouse IL-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Restrictions: For Research Use only

Handling

Storage Comment: Please refer to the Manual

Target Details for IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: IL-4 (Interleukin 4)

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation