IL-7 ELISA Kit

Catalog No. ABIN8126253

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse IL-7.

Quick Overview for IL-7 ELISA Kit (ABIN8126253)

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details IL-7 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: This assay employs an antibody specific for Anti-mouse IL-7 coated on a 96-well plate.

Analytical Method: Quantitative

Sensitivity: 9.4 pg/mL

Characteristics: This immunoassay kit allows for the in vitro quantitative determination of Mouse IL-7 concentrations in serum, plasma.  Detection Range: 15.6 - 1000 pg/mL Sensitivity: < 9.4 pg/mL

Application Details

Plate: Pre-coated

Protocol: This assay employs an antibody specific for Anti-mouse IL-7 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Restrictions: For Research Use only

Handling

Storage Comment: Please refer to the Manual

Target Details for IL-7

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Alternative Name: IL-7 (Interleukin 7)

Pathways: JAK-STAT Signaling