Galectin 3 ELISA Kit
Quick Overview for Galectin 3 ELISA Kit (ABIN8126255)
Target
See all Galectin 3 (LGALS3) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology.
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Analytical Method
- Quantitative
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Characteristics
- This immunoassay kit allows for the in vitro quantitative determination of Porcine Gal 3 (Galectin 3) concentrations in serum, plasma. Detection Range: 0.156-10 ng/mL Sensitivity: <0.094 ng/mL
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Plate
- Pre-coated
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Protocol
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- GAL3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-GAL3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GAL3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GAL3 can be calculated.
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Restrictions
- For Research Use only
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Storage Comment
- Please refer to the Manual
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- Galectin 3 (LGALS3)
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Alternative Name
- GAL3 (Galectin 3)
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Pathways
- RTK Signaling
Target See all Galectin 3 (LGALS3) ELISA Kits
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