GDNF ELISA Kit

Catalog No. ABIN921058

Human GDNF ELISA Kit Colorimetric assay for quantification of Human GDNF and has been mentioned in 7+ publications.

Quick Overview for GDNF ELISA Kit (ABIN921058)

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Binding Specificity: AA 109-211

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details GDNF ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human GDNF

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: R109-I211

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <4pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: R109-I211

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the TGF-beta family. GDNF subfamily.

Tissue Specificity: In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change. .

Plate: Pre-coated

Protocol: human GDNF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GDNF has been precoated onto 96-well plates. Standards(NSO, R109-I211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GDNF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human GDNF amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human GDNF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human GDNF standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 157, Standard deviation: 6.44, CV(%): 4.1
• Sample 2: n=16, Mean(pg/ml): 634, Standard deviation: 36.14, CV(%): 5.7
• Sample 3: n=16, Mean(pg/ml): 1416, Standard deviation: 72.22, CV(%): 5.1,
• Sample 1: n=24, Mean(pg/ml): 144, Standard deviation: 10.66, CV(%): 7.4
• Sample 2: n=24, Mean(pg/ml): 667, Standard deviation: 54.69, CV(%): 8.2
• Sample 3: n=24, Mean(pg/ml): 1424, Standard deviation: 106.8, CV(%): 7.5

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for GDNF ELISA Kit (ABIN921058)

Niu, Li, Wu, Wu, Yan, Shang, Bai, Li, Hua: "Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells." in: Oncotarget, Vol. 7, Issue 47, pp. 77532-77542, (2018) (PubMed).

Guo, Sun, Xu, Zhao, Peng, Wang: "Human umbilical cord mesenchymal stem cells promote peripheral nerve repair via paracrine mechanisms." in: Neural regeneration research, Vol. 10, Issue 4, pp. 651-8, (2015) (PubMed).

Liu, Wang, Shao, Liu: "Genetically modified Schwann cells producing glial cell line-derived neurotrophic factor inhibit neuronal apoptosis in rat spinal cord injury." in: Molecular medicine reports, Vol. 9, Issue 4, pp. 1305-12, (2014) (PubMed).

Banerjee, Nürnberger, Hennerbichler, Riedl, Schuh, Hacobian, Teuschl, Eibl, Redl, Wolbank: "In toto differentiation of human amniotic membrane towards the Schwann cell lineage." in: Cell and tissue banking, Vol. 15, Issue 2, pp. 227-39, (2014) (PubMed).

Chai, Guo, Li, Wang, Wang, Shi, Hu, Liu, Adah: "Scutellarin and caffeic acid ester fraction, active components of Dengzhanxixin injection, upregulate neurotrophins synthesis and release in hypoxia/reoxygenation rat astrocytes." in: Journal of ethnopharmacology, Vol. 150, Issue 1, pp. 100-7, (2013) (PubMed).

Wang, Hou, Yuan, Hou, Liu, Li, Zhang: "Association study between plasma GDNF and cognitive function in late-onset depression." in: Journal of affective disorders, Vol. 132, Issue 3, pp. 418-21, (2011) (PubMed).

Yang, Zhou, Gao, Chen, Tu, Sun, Liu, He, Liu, Yuan: "Neuroprotective effects of bone marrow stem cells overexpressing glial cell line-derived neurotrophic factor on rats with intracerebral hemorrhage and neurons exposed to hypoxia/reoxygenation." in: Neurosurgery, Vol. 68, Issue 3, pp. 691-704, (2011) (PubMed).

Target Details for GDNF

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Alternative Name: GDNF

Background:

Protein Function: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. .

Background: Glial cell line-derived neurotrophic factor(GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily. GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures. GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system. GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons. GDNF lies on the short arm of human chromosome 5, at 5p13.1-p13.3 ability to promote dopamine uptake in midbrain cultures.

Synonyms: Glial cell line-derived neurotrophic factor,hGDNF,Astrocyte-derived trophic factor,ATF,GDNF,

Full Gene Name: Glial cell line-derived neurotrophic factor

Cellular Localisation: Secreted.

Gene ID: 2668

UniProt: P39905

Pathways: RTK Signaling, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration