IL-5 ELISA Kit

Catalog No. ABIN921063

The Human IL-5 ELISA Kit (ABIN921063) is a Colorimetric ELISA Kit designed to quantify Human IL-5. This ELISA Kit has been cited in 5 publications.

Quick Overview for IL-5 ELISA Kit (ABIN921063)

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Binding Specificity: AA 20-134

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.8 pg/mL - 500 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine

Product Details IL-5 ELISA Kit

Minimum Detection Limit: 7.8 pg/mL

Purpose: Sandwich Human IL-5/Interleukin-5 ELISA Kit to quantitate Human IL5 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: I20-S134

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 2 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 7.8%, 6.4%, 6.9%
Inter-Assay Precision (CV%): 9.3%, 6.5%, 8.6%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for IL-5 ELISA Kit (ABIN921063)

Presti, DOrazio, Labra, La Ferla, Mezzasalma, Bizzaro, Giardina, Michelotti, Tursi, Vassallo, Di Gennaro: "Evaluation of the probiotic properties of new Lactobacillus and Bifidobacterium strains and their in vitro effect." in: Applied microbiology and biotechnology, Vol. 99, Issue 13, pp. 5613-26, (2015) (PubMed).

Wang, Deng, Ren, Xiao, Chen, Tao: "Lactoferrin administration into the nostril alleviates murine allergic rhinitis and its mechanisms." in: Scandinavian journal of immunology, Vol. 78, Issue 6, pp. 507-15, (2013) (PubMed).

Chen, Duan, Kang, Yang, Jiang, Zhang, Li, Yin, Hu, Dong, Li, Hajjar, Han: "Activation of liver X receptor induces macrophage interleukin-5 expression." in: The Journal of biological chemistry, Vol. 287, Issue 52, pp. 43340-50, (2012) (PubMed).

Zhu, Qiu, Liu, Dong, Weng, Hu, Kuang, Gao, Dan, Tao: "Tolerogenic dendritic cells generated by RelB silencing using shRNA prevent acute rejection." in: Cellular immunology, Vol. 274, Issue 1-2, pp. 12-8, (2012) (PubMed).

Tong, Bai, Liu, Wang, Zhang, Xiao, Xu, Liu, Yang, Wu: "Expression, purification and monoclonal antibodies preparation of recombinant equine mature interleukin-18." in: Veterinary immunology and immunopathology, Vol. 136, Issue 3-4, pp. 194-200, (2010) (PubMed).

Target Details for IL-5

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Alternative Name: IL5

Background:

Background: Interleukin-5 (IL-5) is also known as eosinophil differentiation factor (EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70 % amino acid sequence homology.

Gene Full Name: interleukin 5

Gene ID: 3567

UniProt: P05113

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour