TNF alpha ELISA Kit

Catalog No. ABIN930377

This ELISA kit is designed for the quantitative measurement of Mouse TNF alpha.

Quick Overview for TNF alpha ELISA Kit (ABIN930377)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Mouse

Application: ELISA

Product Details TNF alpha ELISA Kit

Characteristics: ELISA kit for the detection of TNF alpha in the research laboratory
Alternative Names: Mouse TNF alpha ELISA kit, TNF alpha ELISA kit, TNFalpha ELISA kit, TNFa ELISA kit, TNFa ELISA kit, Tumor Necrosis Factor ELISA kit, TNFSF2 ELISA kit, Cachectin ELISA kit, Differentiation-inducing factor ELISA kit, DIF ELISA kit, Necrosin ELISA kit, Cytotoxin ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end user

Assay Procedure:

This mouse TNFalpha Enzyme Immunometric Assay (EIA) kit is a complete kit for the determintation of mouse TNFalpha in biological fluids. Please read the complete kit insert before performing this assay. The kit uses a monoclonal antibody to mouse TNFalpha immobilized on a microtiter plate to bind the mouse TNFalpha in the standards or sample. A recombinant mouse TNFalpha Standard is provided in the kit. After a short incubation the excess sample or standard is washed out and a polyclonal antibody to mouse TNFalpha is added. This antibody binds to the mouse TNFalpha captured on the plate. After a short incubation the excess antibody is washed out and donkey antirabbitIgG conjugated to Horseradish peroxidase is added, which binds to the polyclonal mouse TNFalpha antibody. Excess conjugate iswashed out and substrate is added.After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration of mouse TNFalpha in either standards or samples.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF alpha

Background: Tumor Necrosis Factoralpha (TNF alpha) is a 17.5 kDa, 157 amino acid protein that is a potent lymphoid factor, which exerts cytotoxic effects on a wide range of tumor cells and other target cells. TNF alpha has been suggested to play a proinflammatory role and has been detected in synovial fluid of patients with rheumatoid arthritis. It is the primary mediator of immune regulation. The biosynthesis of TNF alpha is tightly controlled, being produced in extremely small quantities in quiescent cells, but is a major secreted factor in activated cells.
Synonyms: Mouse TNF alpha ELISA kit, TNF alpha ELISA kit, TNFalpha ELISA kit, TNFa ELISA kit, TNFa ELISA kit, Tumor Necrosis Factor ELISA kit, TNFSF2 ELISA kit, Cachectin ELISA kit, Differentiation-inducing factor ELISA kit, DIF ELISA kit, Necrosin ELISA kit, Cytotoxin ELISA kit.

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome