PDL1 ELISA Matched Antibody Pair (Risk Free)
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- Target See all PD-L1 products
- PD-L1 (CD274 (PD-L1))
- Fragment
- single-domain Antibody (sdAb)
- Binding Specificity
- Extracellular Domain
- Reactivity
- Human
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Host
- Various Hosts
- Clonality
- Monoclonal
- Application
- ELISA
- Brand
- Risk-Free™
- Characteristics
- Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PD-L1 and PD-L2. PD-L1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells. This suggests that PD-L1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts.
- Purification
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Mouse Monoclonal: Antibodies supplied as protein A purified IgG1.
Single Domain: Antibodies supplied as affinity chromatography purified via nickel column. - Components
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Capture: PD-L1 Antibody [4F2]
Detection: PD-L1 Antibody [F6A9] - Immunogen
- PD-L1 antibodies were raised against the extracellular domain of human PD-L1.
- Clone
- 4F2-F6A9
- Isotype
- IgG1
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CD274 (Human) Matched Antibody Pair
ELISA Reactivity: Human ELISA unconjugated Host: Mouse
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- Comment
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Risk-Free™ antibodies are monoclonal antibodies made to improve in vitro studies.
Unlike antibodies developed using proteins made in yeast or bacteria, Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein.
These antibodies have the following advantages:
- Antibodies are developed against mammalian-expressed, post-translationally modified recombinant proteins.
- Multiple antibodies per target allows the user to choose the best antibody for their application
- Available individually or as a set
- Risk-Free™ means they are rigorously tested and guaranteed to work for their designated applications:
- ELISA
- Flow Cytometry
- Immunoblot
- Immunohistochemistry
- Immunocytochemistry
- Immunofluorescence - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS containing 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage Comment
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Mouse Monoclonal: Antibodies can be stored at 4 ˚C for three months and -20 ˚C, stable for up to one year.
Single Domain: Antibody should be stored in working aliquots at -20°C, stable for up to one year.
As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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- Target
- PD-L1 (CD274 (PD-L1))
- Alternative Name
- PDL1 (PD-L1 Products)
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