PDL1 ELISA Matched Antibody Pair (Risk Free)
Quick Overview for PDL1 ELISA Matched Antibody Pair (Risk Free) (ABIN5608627)
Target
Binding Specificity
Reactivity
Host
Clonality
Application
Clone
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Fragment
- single-domain Antibody (sdAb)
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Brand
- Risk-Free™
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Characteristics
- Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PD-L1 and PD-L2. PD-L1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells. This suggests that PD-L1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts.
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Purification
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Mouse Monoclonal: Antibodies supplied as protein A purified IgG1.
Single Domain: Antibodies supplied as affinity chromatography purified via nickel column. -
Components
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Capture: PD-L1 Antibody [8E12]
Detection: PD-L1 Antibody [F6A9] -
Immunogen
- PD-L1 antibodies were raised against the extracellular domain of human PD-L1.
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Isotype
- IgG1
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CD274 (Human) Matched Antibody Pair
ELISA Reactivity: Human ELISA unconjugated Host: Mouse
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Comment
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Risk-Free™ antibodies are monoclonal antibodies made to improve in vitro studies.
Unlike antibodies developed using proteins made in yeast or bacteria, Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein.
These antibodies have the following advantages:
- Antibodies are developed against mammalian-expressed, post-translationally modified recombinant proteins.
- Multiple antibodies per target allows the user to choose the best antibody for their application
- Available individually or as a set
- Risk-Free™ means they are rigorously tested and guaranteed to work for their designated applications:
- ELISA
- Flow Cytometry
- Immunoblot
- Immunohistochemistry
- Immunocytochemistry
- Immunofluorescence -
Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- PBS containing 0.02 % sodium azide.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage Comment
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Mouse Monoclonal: Antibodies can be stored at 4 ˚C for three months and -20 ˚C, stable for up to one year.
Single Domain: Antibody should be stored in working aliquots at -20°C, stable for up to one year.
As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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- PD-L1 (CD274 (PD-L1))
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Alternative Name
- PDL1
Target
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