Quick Overview for DYKDDDDK Tag Peptide (ABIN1607595)
Target
Source
Application
-
-
Peptide Type
- Synthetic
-
Supplier Product No.
- 000-000-383
-
Supplier
- Rockland
-
Purpose
- DYKDDDDK (FLAG®) Peptide
-
Characteristics
- synthetic FLAG® peptide, ddddk peptide, dykddddk peptide, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, control peptide, blocking peptide, DDK, FLAG
-
Purification
- Greater than 90% specific peptide by HPLC and MS.
-
Purity
- Greater than 90% specific peptide by HPLC and MS.
-
-
DYKDDDDK Tag peptide
Host: Synthetic IP, SDS, WB Without preservative
-
-
Application Notes
-
Application Note: The FLAG® peptide was tested by SDS-PAGE. A working concentration of 500 μg/mL for triple FLAG® peptide is typically used for competitive elution of FLAG® containing proteins from anti-DYKDDDDK affinity gels (p/n 200-350-383). For use as a control in antibody-peptide competition assays (PCA) use the peptide at 1.0 μg per 1.0 μL of antibody/antiserum in per assay. See anti-DYKDDDDK FLAG® affinity gel PROTOCOL for specific instructions for use.
Western Blot Dilution: User Optimized as blocking peptide in PCA
Immunoprecipitation Dilution: User Optimized - see PROTOCOL
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Reconstitution
-
Reconstitution Buffer: 0.05 M Tris Chloride, 0.15 M Sodium Chloride, pH 7.4
Reconstitution Volume: 1.0 mL
-
Concentration
- 1 mg/mL
-
Buffer
-
Buffer: None
Stabilizer: None
Preservative: None -
Preservative
- Without preservative
-
Storage
- -20 °C
-
Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
-
Expiry Date
- 6 months
-
-
- DYKDDDDK Tag
-
Target Type
- Tag
-
Background
-
Synonyms: synthetic FLAG® peptide, ddddk peptide, dykddddk peptide, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, control peptide, blocking peptide, DDK, FLAG
Background: The FLAG® peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys or DYKDDDDK) is intended for immunoaffinity chromatography to allow elution under non-denaturing conditions by competitive elution with excess of free FLAG® Peptide.
Target
-
