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CD274 (PD-L1) (C-Term) peptide (Biotin)

PD-L1 Reactivity: Human Host: Synthetic BP, Imm
Catalog No. ABIN5539420
  • Target See all PD-L1 products
    PD-L1 (CD274 (PD-L1))
    Protein Region
    C-Term
    Origin
    Human
    Source
    • 6
    Synthetic
    Purification tag / Conjugate
    This PD-L1 peptide is labelled with Biotin.
    Application
    Blocking Peptide (BP), Immunogen (Imm)
    Sequence
    CKKQSDTHLE ET.
    Predicted Reactivity
    Human
  • Application Notes
    When peptides are reconstituted in 200 μL water, the concentration would be also 0.5 mg/mL. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20 min. Then incubate and develop in parallel the two blots.Approx 40 kDa band observed in Human Heart lysates (calculated MW of 33.3 kDa according to NP_054862.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1 μg/mL.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
    Buffer
    100µg of Lyophilised peptide, formulation De-ionized (DI) water.
    Storage
    -20 °C
    Storage Comment
    Shipped at ambient temperature, store at -20°C.
  • Target
    PD-L1 (CD274 (PD-L1))
    Synonyms
    B7-H Peptide, B7H1 Peptide, PD-L1 Peptide, PDCD1L1 Peptide, PDCD1LG1 Peptide, PDL1 Peptide, A530045L16Rik Peptide, B7h1 Peptide, Pdcd1l1 Peptide, Pdcd1lg1 Peptide, Pdl1 Peptide, RGD1566211 Peptide, CD274 molecule Peptide, CD274 antigen Peptide, programmed cell death 1 ligand 2 Peptide, CD274 Peptide, Cd274 Peptide, PDCD1LG2 Peptide
    Gene ID
    29126
    NCBI Accession
    NP_054862, NP_001254635
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