alpha-conotoxin IMI peptide (Atto 590)
Reactivity: Conus imperialis
Host: Synthetic
BP, FM, IF, LCI
Catalog No. ABIN7582096
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Quick Overview for alpha-conotoxin IMI peptide (Atto 590) (ABIN7582096)
Target
alpha-conotoxin IMI
Origin
Conus imperialis
Source
Synthetic
Application
Blocking Peptide (BP), Fluorescence Microscopy (FM), Immunofluorescence (IF), Live Cell Imaging (LCI)
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Peptide Type
- Synthetic
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Purification tag / Conjugate
- Atto 590
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Purpose
- A Potent Antagonist of Mammalian α3/β2 nAChR Conjugated to the Fluorescent Dye ATTO-590
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Sequence
- GCCSDPRCAW RC-NH2
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Specificity
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Toxin target: α3/β2 nAChR,
Disulfide bonds between: Cys2-Cys8, Cys3-Cys12 Cys12 - C-terminal amidation ATTO Fluor-590 -
Characteristics
- A Potent Antagonist of Mammalian α3/β2 nAChR Conjugated to the Fluorescent Dye
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alpha-conotoxin IMI peptide
Reactivity: Conus imperialis Host: Synthetic BP
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Application Notes
- Antigen Preadsorption Control: 1 - 2 μM
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is lyophilized in 0.5 mL conical vial. The product is soluble in pure water to high-micromolar concentrations (5 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity. Avoid exposure to light.
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Buffer
- Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
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Storage
- -20 °C
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Storage Comment
- Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.,Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.
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- alpha-conotoxin IMI
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Background
- α-CTx ImI, alpha-conotoxin ImI, Alpha-CTx ImI,α-Conotoxin ImI is a 12 amino acid peptidyl toxin isolated from Conus Imperialis venom1, which blocks mammalian neuronal nAChRs located on postsynaptic membranes. This toxin inhibits mammalian neuronal nAChRs (α3/β2 > α7 > α3/β4) in a voltage-independent manner and has no effect on nAChRs composed of α2/β2, α3/β2, α4/β2, α2/β4, α3/β4, or α4/β4 subunits1,2.
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Molecular Weight
- 1924 Da
Target
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