alpha-conotoxin IMI peptide (Atto 590)
Reactivity: Conus imperialis
Host: Synthetic
FM, IF, LCI
Catalog No. ABIN7582096
$710.62
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Quick Overview for alpha-conotoxin IMI peptide (Atto 590) (ABIN7582096)
Target
alpha-conotoxin IMI
Origin
Conus imperialis
Source
Synthetic
Application
Fluorescence Microscopy (FM), Immunofluorescence (IF), Live Cell Imaging (LCI)
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Peptide Type
- Synthetic
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Biological Activity
- Active
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Purification tag / Conjugate
- Atto 590
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Purpose
- α-Conotoxin ImI-ATTO Fluor-590
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Sequence
- GCCSDPRCAW RC-NH2
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Specificity
- Toxin target: α3β2 and α7 nAChRs
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Characteristics
- ATTO 590 (Ex/Em: 593/622 nm). The extent of labeling is 1 Molecule of dye per molecule of i±-Conotoxin ImI, linked via primary amines.
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Biological Activity Comment
- α-Conotoxin ImI potently and specifically blocks mammalian neuronal nAChR (α3/β2 > α7 > α3/β4)1.
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alpha-conotoxin IMI peptide
Reactivity: Conus imperialis Host: Synthetic
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Application Notes
- Antigen Preadsorption Control: 1 - 2 μM
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Note: The product is typically supplied in a 0.5 mL conical vial. The product is readily soluble in high-quality pure water at micromolar to millimolar concentrations (typically 5 μM to 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x the final working concentration. Divide the stock solution into small, single-use aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles and exposure to light to maintain optimal biological activity.
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Buffer
- Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).
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Handling Advice
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid vigorous vortexing. protect from moisture,keep the vial protected from light
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Storage
- -20 °C
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Storage Comment
- The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C, protect from moisture, and keep the vial protected from light.
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- alpha-conotoxin IMI
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Background
- A fluorescent probe for imaging and sensitive detection of α3β2 and α7 nicotinic acetylcholine receptors (nAChR)
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Molecular Weight
- 1924 Da
Target
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