Ubiquitin-Conjugating Enzyme E2N (UBE2N) Peptide
Quick Overview for Ubiquitin-Conjugating Enzyme E2N (UBE2N) Peptide (ABIN8115295)
Target
Origin
Source
Application
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Purpose
- UBE2N Peptide - middlel region
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Sequence
- IRTVLLSIQA LLSAPNPDDP LANDVAEQWK TNEAQAIETA RAWTRLYAMN
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This is a synthetic peptide designed for use in combination with anti- UBE2N Antibody (ARP90265_P050),. It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications. Please inquire for more details.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 µL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
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Buffer
- Lyophilized powder
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Storage
- -20 °C
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Storage Comment
- For longer periods of storage, store at -20 °C. Avoid repeat freeze-thaw cycles.
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- UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))
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Background
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Background Information: The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains (PubMed:22424771, PubMed:28039360). This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes.
Alternative Symbols: UBC13, AL022654, BB101821, 1500026J17Rik
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Molecular Weight
- 16 kDa
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Gene ID
- 93765
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NCBI Accession
- NP_542127
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UniProt
- P61089
Target
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