PPP3R1 Protein (AA 1-170) (His tag)

Catalog No. ABIN1096182

Product Details

Target: PPP3R1 (Protein Phosphatase 3, Regulatory Subunit B, alpha (PPP3R1))

Protein Type: Recombinant

Protein Characteristics: AA 1-170

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PPP3R1 protein is labelled with His tag.

Purpose: Recombinant Human Calcineurin Subunit B1/CNB1 (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MGNEASYPLE MCSHFDADEI KRLGKRFKKL DLDNSGSLSV EEFMSLPELQ QNPLVQRVID IFDTDGNGEV DFKEFIEGVS QFSVKGDKEQ KLRFAFRIYD MDKDGYISNG ELFQVLKMMV GNNLKDTQLQ QIVDKTIINA DKDGDGRISF EEFCAVVGGL DIHKKMVVDV

Characteristics: Recombinant Human Calcineurin Subunit B Type 1/CNB is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Val170) of Human PPP3R1 fused with a 6His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 2 mM DTT, pH 8.0 .

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: PPP3R1 (Protein Phosphatase 3, Regulatory Subunit B, alpha (PPP3R1))

Alternative Name: PPP3R1 (PPP3R1 Products)

Synonyms: si:ch211-274c22.8 Protein, canb Protein, CALNB1 Protein, CNB Protein, CNB1 Protein, CaNB1 Protein, Cnb1 Protein, MCIP1 Protein, protein phosphatase 3, regulatory subunit B, alpha a Protein, protein phosphatase 3 regulatory subunit B, alpha S homeolog Protein, protein phosphatase 3 regulatory subunit B, alpha Protein, protein phosphatase 3, regulatory subunit B, alpha Protein, protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I) Protein, ppp3r1a Protein, ppp3r1.S Protein, PPP3R1 Protein, Ppp3r1 Protein

Sub Type: Fusionprotein

Background: Calcineurin Subunit B Type 1 belongs to the calcineurin regulatory subunit family. Calcineurin Subunit B Type 1 is a Ser/Thr-specific calcium and calmodulin-dependent protein phosphatase. It is composed of a catalytic subunit (A) and a regulatory subunit (B). It contains four EF-hand domains and four functional calcium-binding sites. Calcineurin Subunit B Type 1 plays an improtant role in the T cell activation pathway.
Alternative Names: Calcineurin Subunit B Type 1, Protein Phosphatase 2B Regulatory Subunit 1, Protein Phosphatase 3 Regulatory Subunit B Alpha Osoform 1, PPP3R1, CNA2, CNB

Molecular Weight: 21.46 kDa

UniProt: P63098

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling