PPP3R1 Protein (His tag)

Catalog No. ABIN7317211

This Recombinant PPP3R1 protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for PPP3R1 Protein (His tag) (ABIN7317211)

Target: PPP3R1 (Protein Phosphatase 3, Regulatory Subunit B, alpha (PPP3R1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 87 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This PPP3R1 protein is labelled with His tag.

Purpose: Recombinant Human PPP3R1 Protein (His Tag)(Active)

Sequence: Gly2-Val170

Characteristics: A DNA sequence encoding the human PPP3R1 (P63098) (Gly2-Val170) was fused with a polyhistide tag at the N-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Using the Octet RED System, the affinity constant (Kd) of human PPP3R1-His bound to Human PPIA-His was 6 nM.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 10 % glycerol, 2 mM DTT

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: PPP3R1 (Protein Phosphatase 3, Regulatory Subunit B, alpha (PPP3R1))

Alternative Name: PPP3R1

Background:

Background: PPP3R1 belongs to the calcineurin regulatory subunit family. It is a regulatory subunit of calcineurin. Calcineurin is composed of two subunits: calcineurin A (CnA) and calcineurin B (CnB). Dephosphorylation of the nuclear factor of activated T-cells (NF-AT) by Calcineurin is essential for NF-AT activation, nuclear translocation, and early gene expression in T-cells. PPP3R1 is a Ser/Thr-specific calcium and calmodulin-dependent protein phosphatase which takes a vital part in the T cell activation pathway. PPP3R1 is involved in protein dephosphorylation, NFAT protein import into nucleus (ortholog) and epithelial to mesenchymal transition (ortholog). It participates in calcineurin signaling pathway, mitogen activated protein kinase signaling pathway. PPP3R1 interacts with (+)-pilocarpine, 2,4-dinitrotoluene and ammonium chloride. It contains four EF-hand domains and four functional calcium-binding sites. PPP3R1 play an improtant role in the T cell activation pathway.

Synonym: Calcineurin Subunit B Type 1, Protein Phosphatase 2B Regulatory Subunit 1, Protein Phosphatase 3 Regulatory Subunit B Alpha Osoform 1, PPP3R1, CNA2, CNB

Molecular Weight: 21.4 kDa

UniProt: P63098

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling