C5A Protein (AA 679-755)

Catalog No. ABIN1096418

This Recombinant C5A protein is produced in Escherichia coli (E. coli).

Quick Overview for C5A Protein (AA 679-755) (ABIN1096418)

Target: C5A (C5a) (Complement Component 5a (C5a))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 679-755

Purpose: Recombinant Mouse Complement Component C5

Sequence: MNLHLLRQKI EEQAAKYKHS VPKKCCYDGA RVNFYETCEE RVARVTIGPL CIRAFNECCT IANKIRKESP HKPVQLGR

Characteristics: Recombinant Mouse Complement C5a produced in E. coli is a single non-glycosylated polypeptide chain containing 78 amino acids (N679-R755) with a molecular mass of 9 kDa.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 350 mM NaCl, pH 7.5.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: C5A (C5a) (Complement Component 5a (C5a))

Alternative Name: c5a

Background: Mouse Complement C5 (C5a) is a glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 77 amino acid peptide that is created by the C5a convertase proteolytic cleavage of C5 alphachain in the classical and alternative complement pathway (C4b2a3b, C3bBb3b). Mouse C5a has fouralphahelices, plus three intra-chain disulfide bonds that form a triple loop structure. C5a functions via G-protein coupled receptor (GPCR) (C5aR/CD88). C5a is a potent chemoattractant and anaphylatoxin that acts on all classes of leukocytes and on many other cell types including endothelial, smooth muscle, kidney, liver, and neural cells. It mediates IL-8 release from bronchial epithelial cells. It also triggers an oxidative burst in macrophages and neutrophils, causing release of histamine in basophils and mast cells. C5a anaphylatoxin activity on hepatocytes results indirectly from interaction with nonparenchymal cell via prostanoid secretion. Mouse C5a shares 60% and 82% sequence identity to human and rat C5a, respectively.
Alternative Names: Complement C5, Hemolytic Complement, C5, Hc

Molecular Weight: 9 kDa

UniProt: P06684

Pathways: Complement System, Carbohydrate Homeostasis